Diterpenoid alkaloids from roots of Aconitum kongboense / 中国中药杂志

The chemical constituents from the roots of Aconitum kongboense were studied. Twenty-five diterpenoid alkaloids were isolated from the 95% methanol extract of the roots of A. kongboense by silica gel, reverse-phase silica gel and basic alumina column chromatography. They included a new aconitine-type diterpenoid alkaloid, named as kongboensenine(1), and twenty-four known ones(2-25), i.e., acotarine F(2), acotarine G(3), 14-acetyltalatisamine(4), talatisamine(5), indaconitine(6), yunaconitine(7), chasmanine(8), 6-epi-foresticine(9), homochasmanine(10), 8-deacetyl-yunaconitine(11), chasmaconitine(12), ajaconine(13), franchetine(14), ezochasmanine(15), crassicautine(16), 14-O-deacylcrassicausine(17), genicunine A(18), falconeridine(19), sachaconitine(20), liljestrandisine(21), 8-methyl-14-acetyltalatisamine(22), kongboendine(23), 14-benzoylchasmanine(24) and pseudaconine(25). Their structures were elucidated by common spectroscopic methods including high-resolution electrospray ionization mass spectrometry(HR-ESI-MS) and nuclear magnetic resonance(NMR) techniques. Compounds 2-4, 10, 13, 15-19 and 21-22 were isolated from this plant for the first time. Experimental results showed that all compounds did not have a significant inhibitory activity against acetylcholinesterase(AChE).

Effects and complication treatments of microwave ablation for benign thyroid nodules / 局解手术学杂志

Objective To explore the effects and complication treatments of microwave ablation(MWA) for benign thyroid nodules.Methods The clinical data of 74 patients with benign thyroid nodules treated with MWA in our department from June 2014 to May 2015 were collected and studied retrospectively.Furthermore,the effects and complication treatments were analyzed and calculated.Results A total of 93 nodules were treated with MWA,of which 46 were the main nodules,cystic 47;7 nodules' ablation were not complete,the complete ablation rate was 92.47％.The postoperative reduction of volume in cystic nodules were significantly higher than solid nodules,and the number of solid nodules decreased more rapidly postoperation.The untreated cystic nodules developed a certain degree of growth after 6 months of surgery.After treatment,the TSH of solid nodules were obviously higher,the FT3 and FT4 were much lower than those before treatment and cystic nodules(P ＜ 0.05).A total of 17 cases of complications occurred in the positive symptomatic treatment,all cases were cured within a few days,there was no serious complications.Conclusion Microwave ablation has a high ablation rate in the treatment of thyroid benign nodules,the ablation effects are different between solid and cystic nodules;early and targeted measures can effectively prevent and deal with surgical complication.

Chemical constituents from Hylocereus undatus peels / 中成药

AIM To study the chemical constituents from Hylocereus undatus cv.Vietnam peels.METHODS The ethyl acetate and n-butyl alcohol fractions of 95％ ethanol extract of H.undatus peels were isolated and purified by silica,ODS and semi-preparative HPLC column,then the structures of obtained compounds were identified by spectral data.RESULTS Thirteen compounds were isolated and identified as typhaneoside (1),kaempferol-3-O-β-D-glucopyranosyl-(1→2)-[α-L-rhamnopyranosyl-(1 →6)-β-D-glucopyranoside] (2),quercetin-3-O-neohesperidoside (3),isorhamnetin-3-O-α-L-rhamnopyranosyl (1→2)-β-D-glycopyranoside (4),3'-O-methylquercetin-3-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside (5),benzyl alcohol-β-D-glucopyranoside (6),physcion (7),resveratrol (8),adenosine (9),uridine (10),2-methyl-3-(3 '-indolyl)-propionic acid (11),α-spinasterol (12),β-sitosterol (13).CONCLUSION Compounds 1-12 are isolated from this plant for the first time.

Phenols in Rhododendron primulaeflorum Bur. et Franch / 中国药学杂志

OBJECTIVE: To study the phenols in the young branches and leaves of Rhododendron primulaeflorum Bur. et Franch. METHODS: The constituents were separated and purified by chromatographic methods, and their structures were elucidated by spectroscopic methods and chemical analyses. RESULTS: Eight known phenol compounds, cannabichromeorcinic acid(1), methylorsellinate(2), orsellinic acid(3), orcinol-O-β-D-glucopyranoside(4), 2, 6-dimethoxy-4-hydroxyphenyl-1-O-β-D-glucopyranoside(5), lyoniside(6), 4, 6-dihydroxy-2-O-(β-D-glucopyran-osyl) acetophenone(7) and 3, 5-dihydroxy-4-O-(β-D-glucopyranosyl) acetophenone(8) were isolated from the plant. CONCLUSION: All of the compounds except compound 2 were isolated from this plant for the first time. Copyright 2012 by the Chinese Pharmaceutical Association.

Clinical application research: color doppler flow image and three dimensional color power angiography in the diagnosis of thyroid nodules / 中国地方病学杂志

Objective To evaluate the clinical value of color doppler flow image (CDFI) and three-dimension color power angiography(3D-CPA) in the diagnosis of thyroid nodules. Methods A total of 62 pathologically confirmed thyroid nodules of 30 patients were analyzed. All the patients underwent ultrasound examination and operation in the Second Affiliated Hospital of Harbin Medical University between January, 2008 and May, 2009. Both CDFI and 3D-CPA were performed before the operation. All the nodules were divided into three groups including nodular goiters, thyroid adenomas and thyroid cancers according to the pathological results. The hemodynamic features and the vascular morphology characteristics of nodules in different groups were compared. Results 3D-CPA showed that blood vessels of nodular goiters commonly distributed surrounding the mass and the inner vessels were thin and regular, thyroid adenomas were bulb-shaped and netted structure, and malignant thyroid nodules displayed distorted and irregular distributed vessels. Peak systolic velocity (PSV)of the three groups were (39.43±11.17a), (46.39±12.98) and (65.17±9.23)cm/s, respectively. Resistance index(RI) of the three groups were (0.32±0.08), (0.41±0.06) and (0.69±0.07)cm/s, respectively. Both PSV and RI in malignant thyroid nodules were higher than in nodular goiters and in thyroid adenomas and the difference were statistical significant (all P < 0.05). The blood flow grade of malignant nodules was also higher in malignant nodules than in other two groups(χ2 = 17.11, 12.79, 23.05, 15.41, P< 0.01). Conclusions CDFI and 3D-CPA could visually demonstrate the characteristic and distribution of the inner and outer blood vessels, display the vessels structures, and they are benefit the differential diagnosis of thyroid nodules.